FIG. 2.

Identification of M. avium subsp. paratuberculosis proteins detected by selected MAbs and localization of epitopes to regions of the MAP1643 and MAP3840 gene products. For determination of the approximate locations of MAb epitopes in the primary sequence of these M. avium subsp. paratuberculosis proteins, purified recombinant peptides representing the full length (lane 2), N-terminal half (lane 3), and C-terminal half (lane 4) of each protein were probed by immunoblotting with selected MAbs, as indicated beneath each blot. These results show that MAbs 9G10 and 11F6 react with AceAb and that both MAbs detect an epitope on the C-terminal half of the protein. Similarly, MAbs 11G4 and 13A4 both react with DnaK; however, MAb 13A4 reacts only with the full-length protein and MAb 11G4 detects an epitope on the N-terminal half of DnaK. The blots probed with α-MBP (anti-MBP, a monoclonal antibody developed to the MBP affinity tag) detect all the proteins present and indicate their relative amounts and positions within each blot. The MBP-LacZ control protein is present in lane 5. No reactivity is observed with the protein size standards (lane 1). Kilodalton size standards are indicated in the left margin.