FIG. 4.

Localization of antigens in fractionated M. avium subsp. paratuberculosis cell lysates. Equal amounts (0.5 μg/lane) of cell lysates from membrane-enriched M. avium subsp. paratuberculosis K-10 (lanes M) and cytoplasmic enriched fractions of M. avium subsp. paratuberculosis K-10 (lanes C) were loaded onto SDS-polyacrylamide gels and analyzed by immunoblotting with selected MAbs, which are indicated beneath each blot. Three MAbs detected proteins in the cytoplasmic enriched fraction, and four MAbs detected proteins present in the membrane-enriched fraction. Kilodalton size standards are indicated in the left margin.