TABLE 2.
Oligonucleotide primers used to amplify MAP1643, MAP3840, and MAP0105c
| Primer name | Sequencea | Location within geneb |
|---|---|---|
| MAP1643F | ATCCTCTAGAATCGACAAAGAGACGCAAGTCCG | 10 to 32 |
| MAP1643R | GCGCAAGCTTCAACCCTCTTTGGTGATCAGCTT | 2266 to 2287 |
| MAP1643F2 | ATCCTCTAGACAGTCCATCGACGACCTGTTC | 1021 to 1041 |
| MAP1643R2 | GCGCAAGCTTCACTCGTCGGCGCTCATGTCAGC | 1153 to 1174 |
| MAP3840F | ATCCTCTAGAATGGCTCGTGCGGTCGGTATCGAC | 1 to 24 |
| MAP3840R | GCGCAAGCTTCACTTGGACTCCCGGTCATCGTC | 1849 to 1872 |
| MAP3840F2 | ATCCTCTAGAGACCGCACCCGTCAGCCGTTCAAG | 859 to 882 |
| MAP3840R2 | GCGCAAGCTTCACGGCTGACGGGTGCGGTCCAG | 856 to 876 |
| 218-4 F | CCTCTTCTAGAATGCGCGGAAACCGCAGCGAG | 163 to 183 |
| 218-4 R | GCGCAAGCTTCATTCGGTGAATGCGCGGAC | 1837 to 1856 |
| 218-9 F | CCTCTTCTAGACGCATGCGATCGAACAGT | 1609 to 1629 |
| 218-9 R | GCGCAAGCTTCAGGTGACGGCGCGTTTGAT | 2587 to 2606 |
a
Underlined nucleotides represent restriction endonuclease cut sites. The XbaI recognition sequence is TCTAGA, and AAGCTT is the HindIII recognition site.
b
The numbers represent the nucleotide position within the respective gene. The gene is indicated within the primer name, except that 218-4 F, 218-4 R, 218-9 F, and 218-9 R target MAP0105c.