. 2007 Feb 28;14(5):493–504. doi: 10.1128/CVI.00371-06

TABLE 2.

Precise mapping of the LX1 epitope of the dengue-2 virus NS1 protein with MAbs

Peptide sequence	ELISA reaction (A₄₉₂) to MAb^a:
Peptide sequence	1A12.3	3D1.4	4H3.4	3A5.4	1G5.4-A1-C3
`RPQPTELRY`	0.24	0.07	0.05	0.04	1.15
`QPTELRYSW`	0.30	0.08	0.07	0.05	1.04
`TELRYSWKT`	0.42	0.13	0.12	0.13	1.24
`LRYSWKTWG`	0.19	1.42	1.37	1.70	0.74
`YSWKTWGKA`	1.73	1.75	0.82	1.29	0.42
`WKTWGKAKM`	0.79	0.87	0.08	0.20	0.16
`TWGKAKMLS`	0.03	0.09	0.05	0.01	0.05
`GKAKMLSTE`	0.02	0.06	0.04	0.01	0.04
`AKMLSTELH`	0.09	0.07	0.04	0.03	0.25
`YSWKTWGK`	0.14	1.09	0.70	0.80	0.19
`YSWKTWG`	0.21	2.49	2.10	1.83	0.16
`YSWKTW`	0.15	1.92	0.54	1.22	0.18
`YSWKT`	0.09	0.12	0.09	0.08	0.14
`SWKTWGKA`	0.47	1.19	0.98	1.05	0.20
`WKTWGKA`	0.87	0.97	0.36	0.58	0.18
`KTWGKA`	0.46	0.08	0.09	0.08	0.17
`TWGKA`	0.11	0.12	0.10	0.09	0.09

MAbs, which defined either the LX1 epitope (MAbs 1A12.3, 3D1.4, 4H3.4, and 3A5.4) or multiple ELK/KLE-type motifs (MAb 1G5.4-A1-C3), were diluted to 1/250 to 1/500 and reacted with overlapping synthetic peptides sequentially spanning amino acids 105 to 129 of the D-2V (strains PR159S1 and TR1751) NS1 protein and a set of peptides in which the LX1 epitope (¹¹³YSWKTWGKA¹²¹) was sequentially truncated. A₄₉₂ values of >1.00 for each MAb are underlined, and the peak reactions against each overlapping and truncated synthetic peptide set are shown in boldface.