TABLE 3.
Comparative ELISA and immunoblot reactions of mouse MAbs against the purified D-2V NS1 protein and the AFLX1 peptide
| MAb | Epitopea | ELISA titer (log10t50)b
|
Immunoblot reactionc
|
||
|---|---|---|---|---|---|
| NS1 protein | AFLX1 peptide | NS1 protein | AFLX1 peptide | ||
| 1A12.3 | LX1 | 3.0 | 3.6 | +++ | +++ |
| 3D1.4 | LX1 | 5.0 | 4.5 | +++ | +++ |
| 4H3.4 | LX1 | 4.3 | 3.4 | ++ | ++ |
| 3A5.4 | LX1 | 5.2 | 4.3 | +++ | +++ |
| 1G5.4-A1-C3 | MULTIPLE | 5.1 | 4.9 | +++ | +++ |
| 1H7.4 (control) | LD2 | 6.1 | <1.5 | +++ | |
a
Epitope name (LD2 or LX1) or reaction with multiple (MULTIPLE) epitopes on the D-2V NS1 protein.
b
Reciprocal log10 t50 against the purified D-2V NS1 protein and the AFLX1 peptide.
c
Immunoblot color reaction intensities of the MAbs against the nonreduced forms of the purified D-2V NS1 protein and the AFLX1 peptide, gauged on an arbitrary scale ranging from negative (blank) to +++.