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. 2007 Feb 28;14(5):493–504. doi: 10.1128/CVI.00371-06

TABLE 5.

Antibody reactions generated in human patients with DF and DSS against sets of overlapping and truncated synthetic peptides within the AFLX1 sequence, the AFLX1 peptide, and the purified D-2V NS1 protein

AFLX1 peptide sequencea A492 for sera from three patients each withb:
DF
DSS
Patient 1 Patient 2 Patient 3 Patient 1 Patient 2 Patient 3
    110ELRYSWKTWGKAKMLSTELHC
RPQPTELRY 0.81 0.80 0.83 1.13 1.25 1.45
    QPTELRYSW 0.74 0.74 0.76 1.11 1.21 1.48
        TELRYSWKT 0.82 0.87 0.91 1.43 1.49 1.72
            LRYSWKTWG 0.42 0.48 0.62 0.86 1.16 1.33
                YSWKTWGKA 0.37 0.43 0.50 1.01 1.06 1.22
                    WKTWGKAKM 0.32 0.38 0.42 0.78 0.94 1.02
                        TWGKAKMLS 0.20 0.30 0.34 0.73 0.87 0.95
                            GKAKMLSTE 0.21 0.24 0.35 0.60 0.64 0.83
                                AKMLSTELH 0.43 0.56 0.51 0.77 0.96 1.27
                YSWKTWGK 0.32 0.37 0.43 0.83 0.98 1.12
                YSWKTWG 0.28 0.34 0.48 1.01 1.03 1.03
                YSWKTW 0.20 0.25 0.31 0.69 0.89 0.95
                YSWKT 0.20 0.23 0.27 0.41 0.55 0.81
                    SWKTWGKA 0.26 0.31 0.34 0.72 0.57 0.95
                        WKTWGKA 0.22 0.25 0.32 0.42 0.45 0.97
                            KTWGKA 0.20 0.22 0.35 0.66 0.48 0.88
                                TWGKA 0.27 0.31 0.54 0.87 0.64 1.05
Anti-D-2V NS1 ELISA titer (log10t50) 3.5 3.6 3.6 4.4 4.6 4.6
Anti-AFLX1 peptide ELISA titer (log10t50) 1.6 1.7 1.9 2.4 2.6 2.8
Immunoblot against the AFLX1 peptide ++ ++ +++
a

Peptide sequences of a set of overlapping 9-amino-acid synthetic peptides sequentially spanning the AFLX1 peptide sequence and a set of sequentially truncated (5- to 8-amino-acid) peptides. The ELK-type motifs are underlined, and the two 9-amino-acid peptides containing the LX1 epitope and the core 7-amino-acid LX1 epitope are shown in boldface.

b

Individual serum samples, obtained 4 to 6 days after the onset of symptoms from patients with either DF (n = 3) or DSS (n = 3), were reacted with the sets of synthetic peptides at 1/75 to 1/125 dilutions, the purified D-2V NS1 protein in an ELISA, and the AFLX1 peptide in an ELISA and an immunoblot assay at 1/50 dilutions. The results with the overlapping and truncated peptides are expressed as A492, values of >1.00 are underlined, and the peak reactions with each of these peptide sets are shown in boldface. The ELISA results are expressed as the reciprocal log10 t50, and the immunoblot color reaction intensities are gauged on an arbitrary scale ranging from negative (blank) to +++.