TABLE 1.
Treatmenta | No. of lymphocytes/nodeb | No. of CD4+ lymphocytes/nodec | No. of CD4+ CD25+ lymphocytes/nodec |
---|---|---|---|
Nonvaccinated | 1.2 × 106 | 2.2 × 105 | 5.7 × 104 |
Vaccinated | 1.9 × 106 | 2.1 × 105 | 4.4 × 104 |
Vaccinated and treated with anti-p19 | 0.9 × 106 | 0.1 × 105 | 2.1 × 104 |
Vaccinated and challenged | 6.1 × 106 | 3.7 × 105 | 1.1 × 105 |
Vaccinated, challenged, and treated with anti-p19 | 7.4 × 106 | 5.5 × 105 | 1.3 × 105 |
Eighteen to 24 popliteal and inguinal nodes were collected from 5 to 6 mice per group 8 days after infection. Single-cell suspensions in PBS were obtained, and cells were counted with a Petroff-Hauser counting chamber.
Obtained by multiplying the percentage of lymphocytes by the average number of cells/node.
Obtained by multiplying the percentage of CD4+ or CD4+ CD25+ lymphocytes by the average number of lymphocytes/node.