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Application of percent apoptosis AC, with an AC limit of ≤18%, to assay testing with frozen PBMC after optimal LN2 storage, 8 h exposure to −20°C storage, and storage temperature cycling. (a) Donor M3143 response to VZV antigen response; (b) donor M5020 response to VZV antigen; (c) donor M3143 response to EPI peptide pool; (d) donor M5020 response to EPI peptide pool.
