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Recognition of H5 AIV by MAb 3D4 and guinea pig monospecific serum against rHA1 by Western blotting. AIV H5N1/PR8, H5N2, and H5N3 purified from allantoic fluid by centrifugation were analyzed by SDS-PAGE and immobilized onto a nitrocellulose membrane. No-virus (negative control) H7N1 HA was not recognized by the antiserum (MAb 3D4 and anti-rHA1), which shows the specificities of the antibodies for the H5 subtypes.
