FIG. 5.

Reactivities of capture and detector antibodies in AC-ELISA format. (A) Equal amounts (1,000 TCID₅₀s) of H5N1/PR8, H5N2, and H5N3 were used in the assay. Both MAbs 3D4 and 8B6 could react with the H5 subtypes, whereas MAb 8B6 possessed a stronger capability of binding to live viruses, and use of the combination of the two MAbs gave higher absorbances. (B) Different concentrations of purified IgG (from a 1:10 dilution to a 1:640 dilution; the concentrations of IgG were 3,200 ng, 1,600 ng, 800 ng, 400 ng, 200 ng, 100 ng, and 50 ng per well, respectively) and approximately 200 ng of rHA1 protein per well was used as the detector antibody in the AC-ELISA.