FIG. 1.

(A) Map of the plasmid pDC1-PfEF-1β-PTP. (B) Outline of the PfEF-1β-PTP cassette. The PTP tag consists of protein C, the TEV protease site, and two protein A epitopes. (C) Analysis of expression of native and PTP-tagged PfEF-1β in wild-type (3D7) and transgenic (TR) parasites using the PAP reagent and anti-PfEF-1β antibodies as described in Materials and Methods. Anti-Pfpmt antibodies were used as a control to detect the native Pfpmt enzyme. M, protein marker. The dots indicate degradation products.