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. 2007 Feb 16;6(4):664–673. doi: 10.1128/EC.00308-06

FIG. 1.

FIG. 1.

Tetracycline induction and dose selection. A: Time course viability assay of control and transfected trophozoites for determining the tetracycline dose. Tetracycline at concentrations of 0.5 μg/ml, 1 μg/ml, and 5 μg/ml were added and incubated for 24 h, 48 h and 72 h. B: Semiquantitative RT-PCR analysis for Eh29 expression in 0.5 μg/ml and 1 μg/ml tetracycline-treated trophozoites. The PCR products were separated on (1%) agarose gels and stained with ethidium bromide. Ethidium bromide-stained PCR products were photographed and then images were analyzed densitometrically. PCR products were quantitated and expressed as percentages. Data represent means ± SEM of three independent experiments (P < 0.05). Bar sets: 1, nontransfected trophozoites; 2, uninduced pEhHYG-tetR-O-Reveh29-transfected trophozoites; 3, 24-h tetracycline-induced pEhHYG-tetR-O-Reveh29-transfected trophozoites; 4, 48-h tetracycline-induced pEhHYG-tetR-O-Reveh29-transfected trophozoites. C: Viability of control, transfected control, and antisense eh29-transfected trophozoites in the presence and absence of 1 μg/ml tetracycline was determined using trypan blue exclusion. Bar sets: 1, nontransfected trophozoites; 2, pEhHYG-tetR-O-CAT-transfected control trophozoites; 3, pEhHYG-tetR-O-Reveh29-transfected trophozoites.