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. 2007 Feb 16;6(4):664–673. doi: 10.1128/EC.00308-06

FIG. 2.

FIG. 2.

Expression of the eh29 gene product in different trophozoites. A and B: Semiquantitative RT-PCR analysis. The PCR products from trophozoites without (A) and with (B) 3-h oxidative stress were separated on a (1%) agarose gel and stained with ethidium bromide. Eh29 and actin products are marked. Ethidium bromide-stained PCR products were photographed and then images were analyzed densitometrically. PCR products were quantitated and expressed as percentages with respect to actin band density. Data represent means ± SEM of three independent experiments (P < 0.05). C and D: Immunoblot analysis of the E. histolytica 29-kDa protein. The immunoblot was prepared by resolving amoeba lysates from nontransfected and different transfected trophozoites without (C) and with (D) 3-h oxidative stress. The blot was developed with NICED 11 antibody specific for Eh29 and with fibronectin to confirm equal loading of proteins. Data represent means ± SEM of several trophozoites from three independent experiments (P < 0.05). Bars: 1, nontransfected trophozoites; 2, pEhHYG-tetR-O-CAT-transfected control trophozoites; 3, uninduced pEhHYG-tetR-O-Reveh29-transfected trophozoites; 4, tetracycline-induced pEhHYG-tetR-O-Reveh29-transfected trophozoites.