FIG. 5.

Shamixanthone, emericellin, and dehydroaustinol do not remediate the sporulation defects of ΔtmpA, ΔfluG, and cfwA2 mutants. Strains FGSC26 (wild type [WT]), TGS6 (ΔtmpA), rBN119 (ΔfluG), and CRO1 (cfwA2) were point inoculated and grown for 48 h at 37°C. After this time, sterile paper discs impregnated with 4 mg of each tested compound were placed ∼1 cm from the edge of the colonies, and pictures were taken after 48 or 72 h. a, CLS12 (cfwA⁺) crude extract; b, CLS2 (cfwA2) crude extract; c, shamixanthone; d, emericellin; e, dehydroaustinol. Arrows in C and D indicate areas showing a reduction in proliferation of aerial hyphae; the rectangle in E indicates the region enlarged in F, showing large numbers of white conidiophores around the paper disc.