TABLE 2.
Primers used in this study
| Primer name | Sequenceb |
|---|---|
| (direction)a | |
| A (forward) | TATTAGCTTAGAAAATTAA |
| B (reverse) | GCAAGTTCTTCAGCTTGTTT |
| G1 (forward) | TTAAGGATCCGATGAAATATTAAAAGATTTG |
| G2 (reverse) | CGCCGTCGACCTAGTGCGTTTTTAAGTAGAT |
| G3 (forward) | AAGCCCTTGCAAATGCATCA |
| G4 (forward) | CCTTTATGAACTTCCTCACT |
| G5 (reverse) | TGATTAACTCGACACCAATC |
| G6 (reverse) | AATCTACAGGCGAGCCATGA |
| G7 (forward) | TTAAGGATCCGATGAAATATCAAAAGATTTG |
| G8 (forward) | TTAAGGATCCGATGAAATATTAAATGATTTG |
| G9 (forward) | TTAAGGATCCGATGAAATATTAAAAGATTTG |
| G10 (reverse) | CGCCGTCGACCTAGTGTGTTTTTAAGTAGAT |
| G11 (reverse) | CGCCGTCGACCTAGTGCGTTTTTAAGTAGAT |
| G12 (reverse) | CGCCGTCGACCTAGTGTGTTTTTAAGTAGAC |
a
Primers A and B for emm typing were designed according to the previous description (http://www.cdc.gov/ncidod/biotech/strep/M-ProteinGene_typing.htm). Primers G1 to G12 were designed based on the sequence of accession no. AJ489606.
b
The sequences for recognition by the restriction enzymes are indicated in boldface.