FIG. 3.

Effects of GelE on antibacterial activity of G. mellonella hemolymph. (A) As shown in the acid extract sample of the immunized hemolymph incubated with GelE (GelE + IH), its antibacterial activity was significantly attenuated and close to that of nonimmunized hemolymph (NH). Error bars were too small to express. (B) A Coomassie blue-stained 16.5% tricine SDS-PAGE gel containing purified Gm cecropin and Gm cecropin treated with GelE or SprE. Lane M, standard marker; lane 1, 2 μg of Gm cecropin; lane 2, 2 μg of Gm cecropin treated with GelE; lane 3, 2 μg of Gm cecropin treated with SprE. (C) HPLC chromatograms of the GelE-induced digestion of Gm cecropin. Inset, radial diffusion assay for antibacterial activities of Fs, F1, and intact Gm cecropin (Cec).