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. 2007 Jan 12;75(4):1679–1689. doi: 10.1128/IAI.01581-06

FIG. 1.

FIG. 1.

Genetic organization and analysis of the gene clusters encoding candidate PmrA response regulators in B. cenocepacia J2315 and K56-2. The positions of the genes and the direction of transcription are indicated by arrows. The vertical arrows indicate genes in which pGPΩTp was integrated, and the corresponding strain designation is indicated above each arrow. The BCAL and BCAM gene designations are based on a preliminary annotation of the B. cenocepacia J2315 genome (www.sanger.ac.uk/Projects/B_cenocepacia/). The abbreviated gene designations are as follows: reg, response regulator; kin, sensor kinase; orf, open reading frame. (A) Four-gene cluster located on chromosome 2 containing genes BCAM2160 to BCAM2163. (B) Six-gene operon located on chromosome 1 containing genes BCAL2831 to BCAL2826. The bars indicate the regions analyzed by RT-PCR as shown in panel C. (C) RT-PCR analysis of the intergenic regions for genes BCAL2831 to BCAL2826. Lanes 1 to 3, DNA with no RT and RT for BCAL2830 and BCAL2829; lanes 4 to 6, DNA with no RT and RT for BCAL2829 and BCAL2828; lanes 7 to 9, DNA with no RT and RT for BCAL2828 and BCAL2827; lanes 10 to 12, DNA with no RT and RT for BCAL2827 and BCAL2826. The arrows indicate bands at expected positions.