FIG. 3.

Effects of AgB and SHF during the differentiation of human monocytes into DCs on the ability to induce a proliferative response. Six-day human monocytes that were differentiated with AgB (10 μg/ml), SHF (50 μg/ml), and LPS (100 ng/ml) or were not stimulated were extensively washed and cultured with allogeneic T lymphocytes (1 × 10⁵ cells/well) for 3 days at different stimulator/responder ratios (DC/T cell ratio, 1:4 to 1:64). Proliferation of allogeneic T cells was measured by determining [methyl-³H]thymidine incorporation. The data are means ± standard deviations for triplicate cultures. The results of one of three experiments are shown; comparable results were obtained in the other experiments.