FIG. 4.

Effects of AgB and SHF on cytokine production by differentiated DCs. Monocytes were induced to differentiate to immature DCs in 6-day cultures with or without AgB (10 μg/ml) or SHF (50 μg/ml). The immature DCs were stimulated with LPS for an additional 18 h, and then cytokine production was analyzed. Cytokine concentrations in culture supernatants were determined by ELISA. The data are means ± standard deviations for seven independent experiments. Significant differences for comparisons with LPS-stimulated DCs as determined by Student's paired t test are indicated by asterisks (P = 0.01), a dagger (P = 0.04), a double dagger (P < 10⁻⁴), and a number sign (P = 0.002).