TABLE 1.

Phenotypic characterization of AgB- and SHF-differentiated cells not stimulated and stimulated with LPS^a

Stimulus	GM-CSF + IL-4	% of cells positive for CD83	Fluorescence intensities for:
			CD40	CD80	CD86	HLA-DR
None	−	3.2 ± 1.1	0.0 ± 0.0	0.0 ± 0.0	25.0 ± 10.0	83.2 ± 21.2
None	+	2.6 ± 1.8	30.1 ± 25.5	38.7 ± 31.1	19.2 ± 8.8	97.1 ± 67.1
SHF	+	10.6 ± 3.4	31.7 ± 29.4	41.4 ± 7.3	55.4 ± 24.6b	80.5 ± 51.1
AgB	+	8.2 ± 2.5	26.8 ± 26.2	34.1 ± 17.6	35.1 ± 16.7c	66.1 ± 51.4
LPS	+	73.5 ± 18.4	48.8 ± 24.8	121.6 ± 59.7	156.6 ± 71.5	119.6 ± 49.3
SHF + LPS	+	12.4 ± 4.2d	48.6 ± 19.3	82.4 ± 55.3d	53.4 ± 23.4d	35.8 ± 14.2d
AgB + LPS	+	4.5 ± 2.2d	43.1 ± 17.3	84.5 ± 73.7d	13.8 ± 5.1d	51.2 ± 18.1d

^aMonocytes were induced to differentiate to immature DCs in 6-day cultures with or without AgB (10 μg/ml) or SHF (50 μg/ml). The immature DCs were stimulated with LPS for an additional 18 h, and surface markers were analyzed by flow cytometry. The results are expressed as the percentage of cells positive for CD83 and as the fluorescence intensities for CD14, CD1a, CD40, CD80, CD86, and HLA-DR; the data are means ± standard deviations for seven independent experiments.

^bSignificantly different (P = 0.003) than the value for unstimulated iDCs as determined by Student's paired t test.

^cSignificantly different (P = 0.014) than the value for unstimulated iDCs as determined by Student's paired t test.

^dSignificantly different (P < 10⁻⁴) than the value for LPS-stimulated DCs as determined by Student's paired t test.