TABLE 3.
Chemokine or cytokine | Avg relative expression (range) ± SD in:
|
||||||||
---|---|---|---|---|---|---|---|---|---|
Spleen
|
Liver
|
MLN
|
|||||||
1 dpi | 2 dpi | 3 dpi | 1 dpi | 2 dpi | 3 dpi | 1 dpi | 2 dpi | 3 dpi | |
IFN-γ | 4.20 (3.1-5.8) | 12.38 (5.0-30.9) | 129.8 (56.1-300.3) | 0.62 (0.3-1.4) | 1.68 (0.5-5.3) | 4.44 (0.2-128.9) | 0.97 (0.6-1.6) | 7.84 (2.3-26.5) | 4.96 (1.2-25.3) |
IL-1β | 2.79 (1.3-6.1) | 8.46 (7.1-10.1) | 16.11 (9.3-27.9) | 4.53 (1.8-11.2) | 1.27 (0.2-10.3) | 1.32 (0.4-4.2) | 5.66 (0.7-46.9) | 72 (10.0-519.1) | 1.62 (0.8-3.5) |
IL-6 | 5.35 (1.0-27.5) | 28.84 (26.4-31.6) | 26.72 (10.4-68.6) | 3.2 (1.9-5.5) | 0.68 (0.1-7.9) | 0.12 (0.01-1.3) | 10.6 (0.7-153.28) | 259.6 (15.3-4389.9) | 4.79 (1.8-12.8) |
TNF-α | 1.28 (1.0-1.6) | 1.64 (1.1-2.4) | 12.38 (7.4-20.7) | 1.2 (0.6-2.2) | 3.48 (0.8-14.3) | 3.56 (0.3-40.5) | 0.98 (0.7-0.4) | 1.11 (0.4-1.9) | 0.52 (0.4-0.7) |
MIP-1α | 1.34 (1.0-1.8) | 7.21 (3.6-14.3) | 70.03 (51.3-95.7) | 1.16 (0.7-1.9) | 2.71 (0.9-8.6) | 4.44 (0.3-63.6) | 1.11 (0.5-2.3) | 5.06 (1.1-23.6) | 1.28 (0.7-2.4) |
MIP-1β | 1.12 (0.7-1.8) | 2.17 (1.4-3.4) | 12.73 (3.8-43.1) | 1.61 (1.0-2.6) | 1.79 (0.9-3.6) | 3.36 (0.5-23.8) | 1.46 (0.6-3.4) | 4.66 (2.8-7.8) | 1.74 (1.0-3.18) |
MIP-2 | 1.12 (0.5-2.4) | 11.63 (8.9-15.2) | 14.32 (9.8-19.6) | 2.39 (1.6-3.6) | 1.21 (0.7-1.9) | 0.38 (0.1-1.5) | 8.22 (0.5-145.0) | 34.54 (4.5-268.7) | 1.69 (0.7-3.97) |
IP-10 | 2.38 (1.5-3.7) | 2.30 (1.1-6.1) | 17.51 (3.8-80.4) | 1.49 (0.7-3.1) | 13.27 (1.0-181.0) | 7.01 (0.4-122.8) | 0.84 (0.6-1.2) | 2.27 (0.4-13.5) | 5.54 (1.9-15.7) |
MCP-1 | 1.55 (0.7-3.5) | 15.35 (12.1-19.4) | 18 (12.0-27.1) | 2.45 (1.6-3.8) | 0.43 (0.04-4.1) | 0.14 (0.02-1.12) | 3.56 (0.2-72.5) | 25.3 (2.6-245.6) | 1.42 (0.8-2.5) |
Groups of mice (n = 5) were inoculated by gavage with 2 × 107 CFU of type A F. tularensis strain FSC033 at day 0, and the MLN, liver, and spleen were collected from each animal at 0, 1, 2, and 3 dpi. The relative levels of cytokine and chemokine mRNA expression were determined by real-time PCR analysis as described in Materials and Methods. Mouse β2-microglobulin RNA was measured and used to calculate relative expression using the formula Rel Exp = 2−ΔΔCt. The results shown are the average of relative expression values determined using cDNA from three mice, with ranges indicated in parentheses.