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Restoration of the reading frame affects uspA2H mRNA levels. RNAs prepared from different M. catarrhalis strains were subjected to Northern blot analysis with probe 2 from Fig. 3. Lanes: 1, O46E parent strain; 2, uspA2H variant O46E.U2V; 3, O46E.U2V-8A; 4, O46E.U2V-XXG. A DNA molecular size marker is indicated on the left.
