FIG. 8.

Determination of the half-life of uspA2H mRNA. Rifampin was added to cells of M. catarrhalis strains O46E and O46E.U2V in the logarithmic phase of growth. RNA prepared from samples of these cells harvested at five different time points was used in a Northern blot analysis with probe 2 from Fig. 3. Three independent experiments were performed to obtain densitometric measurements; the results of one of these experiments are depicted here. DNA molecular size markers are indicated on the left.