TABLE 3.
Expression of β-galactosidase activity by uspA2H-lacZ fusion constructs in E. coli XL10-Gold
| Plasmid | Primers/templatea | Miller unitsb | % Relative to pWW105 |
|---|---|---|---|
| pAC7 | 0 | 0 | |
| pWW105 | 36,959 ± 4,681 | 100 | |
| pWW106 | 201 ± 89 | 0.5 | |
| pWW105.1 | WW102-WW103/pWW105 | 214 ± 19 | 0.6 |
| pWW105.2 | WW104-WW105/pWW105 | 34,200 ± 7,389 | 92.5 |
| pWW105.3 | WW106-WW107/pWW105 | 33,643 ± 5,434 | 91.0 |
| pWW105.12 | WW108-WW109/pWW105.2 | 200 ± 12 | 0.5 |
| pWW105.13 | WW102-WW103/pWW105.3 | 184 ± 16 | 0.5 |
| pWW105.23 | WW104-WW105/pWW105.3 | 33,037 ± 5,871 | 89.3 |
| pWW105.123 | WW126-WW127/pWW105.13 | 130 ± 30 | 0.4 |
| pWW105.M | WW112-WW113/pWW105 | 31,110 ± 5,378 | 84.2 |
| pWW106.M | WW112-WW113/pWW106 | 110 ± 25 | 0.3 |
a
Oligonucleotide primers and template used for mutagenesis of selected codons.
b
Miller units were calculated with the following formula: 1,000 × A420/[time (minutes) × vol (milliliters) × A600]. This formula was modified from that described by Sambrook et al. (35). These values represent the mean ± the standard deviation.