Immunofluorescence localization of spermatozoa incubated with labeled rabbit antiserum against rUreG or NASP393-408. Smeared spermatozoa were labeled with either antiserum to rUreG (1:50) (a and d), anti-NASP393-408 antiserum (1:50) (b and e), or adjuvant control serum (c and f). Human spermatozoa (a, b, and c) and mouse spermatozoa (d, e, and f) are shown. Bar, 5 μm.