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. 2007 Jan 8;75(4):1954–1963. doi: 10.1128/IAI.01034-06

FIG. 1.

FIG. 1.

Effect of increasing numbers of PLCs from F. gigantica-infected ITT sheep on the killing of NEJ F. gigantica. Each of three replicate wells containing 20 to 30 NEJ parasites and PLCs (E/T ratio of 0.25 × 105 to 2 × 105 cells to 1 NEJ parasite) in 1 ml of medium was incubated for 3 days. The viability of the NEJ parasites was then assessed as the ability to reduce the tetrazolium salt MTT. Results are the mean ± the standard deviation of three experiments. Asterisks indicate mean values that are significantly different by the Dunnett's multiple comparison test at P < 0.05, relative to control incubations (cells plus NEJ parasites alone). NS, serum from F. gigantica-naive ITT sheep; IS, immune serum from ITT sheep infected for 8 weeks with F. gigantica.