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Competition binding of PA peptides to HLA DRB1*1302. Six PA peptides and a positive control (HA 306-317) were each incubated with purified recombinant HLA DRB1*1302 at the concentration shown and then challenged with 0.1 μM biotinylated HA 306-318. The DRB1 molecules were captured with anti-MHC monoclonal antibodies on an enzyme-linked immunosorbent assay plate, followed by the addition of europium-labeled streptavidin, and developed using europium activation buffer.
