TABLE 1.
IC50 of PA peptide binding to HLA class II moleculesa
| Peptideb | Result for HLA DRB1 molecule tested
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| DR*0101 | DR*0301 | DR*0401 | DR*0404 | DR*0405 | DR*0701 | DR*1101 | DR*1302 | DR*1501 | |
| PA 112-127 | N | N | N | — | — | — | — | N | — |
| PA 373-393 | 0.25 | 8 | N | — | — | 0.1 | >10 | 0.2 | — |
| PA 374-385 | 5 | N | N | 6.6 | N | 0.08 | N | N | N |
| PA 382-392 | N | N | N | 6.4 | N | N | >10 | 2 | 1.8 |
| PA 579-591 | — | 5 | N | — | — | — | 5 | >10 | — |
| PA 595-605 | N | N | 0.76 | 5 | N | N | N | N | N |
| Positive control | 0.25 | >10 | 0.5 | 0.01 | 1 | 10 | 1 | 10 | 0.225 |
| Positive control peptide | HA | Rubella | HA | Gad5571 | HA | HA | HA | HA | MBP |
a
Values for 50% inhibitory concentration (IC50) are given in μM peptide. N, no binding; —, no suitable motif/not tested.
b
Peptide sequences: PA 112-127, RLYQIKIQYQRENPTE; PA 373-393, PIYNVLPTTSLVLGKNQTLAT; PA 374-385, IYNVLPTTSLVL; PA 381-392, SLVLGKNQTLAT; PA 579-591, DKIKLNAKMNILI; PA 595-605, RFKYDRNNIAV; HA 306- 318, PKYVKQNTLKLAT; Rubella 254-266, RLRLVDADDPLLR; Gad65 555-567 (557I), NFIRMVISNPAAT; MBP 87-96, VHFFKNIVTP.