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. 2007 Feb 5;75(4):1820–1826. doi: 10.1128/IAI.00516-06

FIG. 5.

FIG. 5.

(A) TNF-α production by peritoneal macrophages of C57BL/6 mice or C57BL/6 TLR2 KO mice stimulated with LPS, NS, or OGex A, B, C, or D. The concentration of LPS and OGex A, B, C, and D was 100 ng/ml. Peritoneal adherent cells were stimulated for 18 h with inducers, and the amounts of TNF-α in cell culture supernatants were tested by ELISA. The data are representative of two experiments using four to five mice each. The experiment was repeated twice. (B) RAW 264.7 cells were preincubated with NS, isotype control antibody (Ab), or anti-mouse CD14 MAb (10 μg/ml) for 1 h. Then, they were stimulated with LPS (10 ng/ml), MALP-2, or OGex A, B, C, or D (20 ng/ml) for 18 h. (C) Peritoneal macrophages from C57BL/6 or C57BL/6 CD14 KO mice were stimulated with 50 ng/ml LPS, MALP-2, or OGex A, B, C, or D, and culture supernatants were tested for the presence of TNF-α. Representative data from two similar experiments using four to five mice each are shown.