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. 2007 Feb 5;75(4):1635–1642. doi: 10.1128/IAI.00864-06

FIG. 3.

FIG. 3.

Specificity of anti-CPBAg1 serum. Proteins were separated on a 12% SDS-polyacrylamide gel, and a transfer blot was probed with either anti-CPBAg1 rabbit serum or naive rabbit serum (data not shown) at a dilution of 1:500. Lane 1, CPBAg1 recombinant protein (0.1 ng); lane 2, midgut protein extract from blood-fed mosquito (24 h PBM, 1 μg); lane 3, CPBAg2 recombinant protein (20 ng). The anti-CPBAg1 serum, unlike the nonimmune rabbit serum, recognizes two midgut proteins corresponding to the zymogen (48.2-kDa) and mature (37-kDa) forms of CPBAg1 and to the zymogen form of CPBAg2. MW, molecular weight (in thousands).