FIG. 4.

Effect of anti-CPBAg1 serum on P. falciparum development in A. gambiae. A. gambiae mosquitoes were fed on P. falciparum-infected red blood cells supplemented with either undiluted or diluted (1:1) anti-CPBAg1 serum. The proportion of infected mosquitoes (A) and intensity of infection (B) were determined on day 7 postfeeding. The proportion of infected mosquitoes was determined by using the mean value from each series of four infections using anti-CPBAg1 serum from two different rabbits. Each infection experiment was performed with gametocytes from a single carrier. The frequency distribution of oocysts pooled from four independent assays is displayed, with bars indicating the percentage of mosquitoes with an oocyst number in the range indicated on the x axis. Asterisks indicate statistically significant differences at the 95% confidence level, based on the P value from the chi-square test (A) and ANOVA (B). Numbers above bars (A) indicate the sample size.