FIG. 6.

P. berghei transmission-blocking assays. The development of P. berghei was monitored in A. gambiae mosquitoes fed on control mice and mice immunized with CPBAg1. Data are expressed as the rate of infected mosquitoes determined by counting oocysts on mosquito midguts on day 9 postfeeding (A) and rate of infection (B). Group 1 mice received a single injection and group 2 mice a booster injection as shown in Fig. 5A. The percent reduction in rate of infection was calculated as 100 × [1 − (% infected mosquitoes fed on immunized mice/% infected mosquitoes fed on control mice)]. All data were statistically significant (P < 0.05) compared to the mean value from the control group, determined by the chi-square test.