TABLE 1.
Antibody staining panels
Panel no. | Fluorochrome label
|
Immunophenotypes detected | |||
---|---|---|---|---|---|
FITC | Phycoerythrin | PerCPa | APC | ||
1 | Lineage cocktail | CD83 | HLA-DR | CD11c | DCs and activated monocytes |
2 | GFP beads | CD14 | HLA-DR | CD11c | Monocytes and DCs with internalized beads |
3 | CD14 | CD40 | CD19 | Monocytes and activated B cells | |
4 | CD56 or CD57 | CD69 | CD3 | CD4 | Activated T, NK, and NKT cells |
5 | CD56 or CD57 | CD69 | CD3 | IFN-γ | IFN-γ-producing T, NK, and NKT cells |
6 | γδ T-cell receptor | CD8 | CD3 or CD21 | IFN-γ | IFN-γ-producing T-cell subsets |
PerCP, peridinin chlorophyll protein.