TABLE 1.

Bacterial species-specific primers used in PCR

Bacterium	Primer sequence (5′ to 3′)	Strand	Size (bp)	Conditions
				Melting temp (°C)	No. of cycles
Porphyromonas gingivalis	5′ GGT AAG TCA GCG GTG AAA CC 3′	+	600	55	40
	5′ ACG TCA TCC ACA CCT TCC TC 3′	−
Treponema denticola	5′ TAA TAC CGA ATG TGC TCA TTT ACA T 3′	+	860	62	40
	5′ CTG CCA TAT CTC TAT GTC ATT GCT CTT 3′	−
Tannerella forsythia	5′ AAA ACA GGG GTT CCG CAT GG 3′	+	426	55	40
	5′ TTC ACC GCG GAC TTA ACA GC 3′	−
Fusobacterium nucleatum	5′AGA GTT TGA TCC TGG CTC AG 3′	+	360	60	35
	5′ GTC ATC GTG CAC ACA GAA TTG CTG 3′	−