TABLE 1.
Oligonucleotides used in this study
| Primer | Sequence (5′ to 3 ′) | Gene target |
|---|---|---|
| O1 | GGGATCGAATTCTTAGGGACTGTTTTATCTGCGGCG | cylE |
| O2 | GCGGCAGCACCCGGGCGTGCCAGTTAAAGGCCTACCGTCTGG | cylE |
| O3 | GGCACGCCCGGGTGCTGCCGCATCCGCCAAGGGGAACATGTC | cylE |
| O4 | ATAAGGATCCAAAGCTTTATGACTAGCCAACC | cylE |
| O5 | TTCAGCAGAATTCCTCTCTCGTCAAGCATTGGA | cylJ |
| O6 | GCGGCAGCACCCGGGCGTGCCTCCAAGGATATGAATATCATGTCC | cylJ |
| O7 | GGCACGCCCGGGTGCTGCCGCGCGGAGGCAGAAATTGAATCCTTT | cylJ |
| O8 | CTATAGGGATCCCACATTTATAGGAAATTTC | cylJ |
| O9 | GCTTTGGGAATTCATTTAAACGAGATTGGGTGGA | cylK |
| O10 | GCGGCAGCACCCGGGCGTGCCCTCCAATGTAGACTGCCTATT | cylK |
| O11 | GGCACGCCCGGGTGCTGCCGCGATGGCTATATTTATGGTTATGCT | cylK |
| O12 | AGTAAGGATCCCGTCTCTTTAATGCGG | cylK |
| O13 | GTTTAGAATTCTTAGGCTTACTAACTTAGC | Pcyl |
| O14 | CATTATTATGTTAAAATAGTAAC | Pcyl |
| O15 | GTTACTATTTTAACATAATAATGTGTTTGAAGTAGATGTTTAGA | Pcyl |
| O16 | TGTTAGGATCCACGACACTGCCATCAGCAC | Pcyl |
| O17 | GTAAAGGGATCCGCTGTCTTGAAAGAGGCTATG | cylK |
| O18 | AAGTATCTGCAGACTTAGCACTATTCGCATCA | cylK |