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Antibody recognition of mutant epsilon-toxin. E. coli lysates containing recombinant His6-tagged Etx-H106P (lane 1) and recombinant His6-tagged Etx-H106P Δ(134-145) (lane 2) were immunoblotted with anti-His6 (A), with anti-epsilon-toxin 4D7 (B), or with anti-epsilon-toxin 5B7 (C).
