FIG. 3.

T. cruzi infection induces a higher IL-10/TNF-α ratio in monocytes from indeterminate but not from cardiac chagasic patients. Analysis of in vitro infection (10 parasites/monocyte) influences on the cytokine ratio in monocytes from indeterminate (I; n, 11) and cardiac (C; n, 13) chagasic patients and noninfected individuals (N; n, 8). Cells were double stained for CD14 and IL-10 and TNF-α and analyzed by flow cytometry. Clear and black bars represent the averages ± standard errors obtained by the individual ratios of IL-10/TNF-α in nonstimulated and infected cultures, respectively. Identical numbers above the bars indicate statistical significance values using the Tukey-Kramer test, as described in Materials and Methods.