TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| DH5α | Host E. coli strain for cloning experiments | Stratagene |
| TOP10 | Host E. coli strain for pCR Blunt II TOPO | Invitrogen |
| 86-24 | Stx2+ EHEC strain (serotype O157:H7) | 18 |
| VS145 | 86-24 qseA mutant strain | 58 |
| VS151 | VS145 with plasmid pVS150 | 58 |
| MC4100 | araD139 Δ(araABC-leu)7679 galU galK Δ(lac)X74 rpsL thi | 55 |
| FS02 | MC4100 qseA mutant strain | This study |
| FS76 | FS02 with plasmid pVS150 | This study |
| Plasmids | ||
| pRS551 | lacZ reporter gene fusion vector | 56 |
| pVS143 | qseA::cat cloned into pCVD442 | 58 |
| pBADMycHis | C-terminal Myc-His tag cloning vector | Invitrogen |
| pBlueScriptII | Cloning vector | Stratagene |
| pVS241 | qseA in pBADMycHisA | 57 |
| pVS150 | qseA in pACYC177 | 58 |
| pVS232Z | LEE1::lacZ in pRS551, base pairs −393 to +323 | 60 |
| pVS200 | ler::lacZ in pRS551, base pairs −393 to −42 | This study |
| pVS204 | ler::lacZ in pRS551, base pairs −343 to +86 | This study |
| pVS206 | ler::lacZ in pRS551, base pairs −218 to +86 | This study |
| pVS224 | ler::lacZ in pRS551, base pairs −173 to +86 | This study |
| pVS225 | ler::lacZ in pRS551, base pairs −123 to +86 | This study |