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. 2007 Feb 26;75(5):2189–2200. doi: 10.1128/IAI.01546-06

FIG. 2.

FIG. 2.

Effect of chimeric StxB1-H on Stx2 secretion in EHEC 86-24. Each 2 μg of protein of the cell-associated fraction (P) and an equal volume of the supernatant fraction (S) were analyzed by SDS-PAGE and immunoblotting using a mixture of anti-Stx2 antiserum and anti-His tag antibody. Values represent the ratios of StxA2 protein levels to the cell-associated fraction in the supernatant fraction (upper) and the ratios of StxB-H protein levels to the supernatant fraction in the cell-associated fraction (lower).