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. 2007 Feb 26;75(5):2189–2200. doi: 10.1128/IAI.01546-06

FIG. 5.

FIG. 5.

Effect of StxB2-H (regions A and B) on Stx2 secretion in EHEC 86-24 (A) and size determination of StxB2-H (regions A and B) by gel filtration (B). (A) Two micrograms of protein of the cell-associated fraction (P) and an equal volume of the supernatant fraction (S) were analyzed by SDS-PAGE and immunoblotting using a mixture of anti-Stx2 antiserum and anti-His tag antibody. Values represent the ratios of StxA2 protein levels to the cell-associated fraction in the supernatant fraction (upper) and the ratios of StxB-H protein levels to the supernatant fraction in the cell-associated fraction (lower). (B) Purified StxB2-H (regions A and B) was loaded onto a TSK-G3000SW column and measured by reading the absorbance at 280 nm. Closed arrows indicate the elution of molecular mass standards. The fraction (shaded arrow) of StxB2-H contained the binding activity towards Vero cells, but the fraction (opened arrow) of StxB2-H (regions A and B) showed no binding activity.