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. 2007 Mar 5;75(5):2531–2539. doi: 10.1128/IAI.01866-06

FIG. 5.

FIG. 5.

Western blot analysis of caspase 9 activation. (A) Whole-cell lysates were separated by SDS-PAGE and analyzed by immunoblotting with an anti-caspase 9 antibody, which recognizes the full-length, inactive form of caspase 9 (47 kDa) and the two fragments resulting from cleavage during activation (37 and 35 kDa). Lane 1, uninfected HeLa cells; lane 2, uninfected HeLa cells with STS treatment; lane 3, 2457T-infected cells; lane 4, 2457T-infected cells with STS treatment; lane 5, BS611-infected cells; lane 6, BS611-infected cells with STS treatment. (B) Results of densitometry analysis of the percentage of the total caspase 9 detected in the Western blot are divided into the inactive form (47 kDa) and the two activated forms (37 and 35 kDa). The average percentage of each form is shown, with error bars representing the standard deviations from three independent experiments.