TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| S. flexneri strains | ||
| 2457T | Wild-type serotype 2a | 11 |
| M90T | Wild-type serotype 5 | 23 |
| BS543 | 2457T/ΔicsA | 29 |
| BS547 | 2457T/ΔmxiM1::aphA3 | 31 |
| BS567 | M90T/ΔipaB2::aphA3 | 23 |
| BS611 | 2457T/ΔmxiE2::aphA3 | 15 |
| BS758 | BS543/ΔmxiE2::aphA3 | This study |
| BS766 | 2457T transformed with pKM208 | This study |
| BS826 | 2457T/ΔipgD | This study |
| BS828 | BS543/ΔipgD | This study |
| Plasmids | ||
| pKD3 | oriR6K bla cat | 7 |
| pKM208 | Temperature-sensitive red-, gam-, lacI-expressing plasmid driven by Ptac promoter; bla | 7 |
| pCP20 | FLP+ λ cI857+ λ ρR Reptsbla cat | 6 |