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Primers used in this study
| Purpose | Forward primer
|
Reverse primer
|
||
|---|---|---|---|---|
| Designation | Sequence | Designation | Sequence | |
| Amplify cat cassette for ipgD deletion | ipgDcatF | ACTAATTTGGGATTGCATCAGGTTTCATTTCAAAGCGGAGATTCCTATAAAGTGTAGGCTGGAGCTGCTTC | ipgDcatR | GACGAATACCCTTTCACCATATTCCATATTTTTGGGTCCCCTATTCTTTCCATATGAATACCTCCTTAGT |
| Confirm ipgD deletion | ipgDF | AGAGAACCCTGTTGAATAAG | ipgDR | ATTAGCACATCATCATCAAG |
| ipgDcenF | TTATATCAGCCTATGGATTG | ipgDR | ATTAGCACATCATCATCAAG | |
