TABLE 3.
Cell counts of apoptosis assay experiments
| Expt | No. of cells
|
|||
|---|---|---|---|---|
| Infected
|
Uninfected
|
|||
| Healthyb | Apoptoticc | Healthyb | Apoptoticc | |
| Firsta | 102d | 0 | 0 | 219 |
| Second | 125d | 0 | 0 | 224 |
| Third | 89d | 0 | 0 | 224 |
| Avg | 105d | 0 | 0 | 222 |
a
At least 300 cells were analyzed for each experiment.
b
Defined as having a normal appearance of the nuclei (normal shape and visible nucleolus) in addition to having a normal appearance of the cytoplasm compared to control HeLa cells without STS treatment.
c
Defined as having a characteristic apoptotic nucleus (DNA fragmentation and chromatin condensation) in addition to having cytoplasmic shrinkage compared to control HeLa cells with STS treatment.
d
P value of <0.001 using Fisher's exact test. Results for the three separate experiments were combined using the method of Fisher (10).