FIG. 1.

Immunogenicity of LT delivered by TCI. Following pretreatment of the skin with a nonwoven abrasive EKG prep pad to disrupt the stratum corneum, a wet patch containing 50 μg of one of three lots of LT was applied to the pretreated area on days 0 and 21. Serum samples obtained on days 0, 21, 42, 90, and 381 were analyzed to determine the presence of anti-LT IgG and IgA by ELISA. There were no significant differences between the anti-LT immune responses of the three groups (P < 0.05). The serum antibody levels for the combined treatment groups are expressed in ELISA units; the bars indicate geometric means, and the error bars indicate 95% confidence intervals. The percentages are percentages of seroconversion (a twofold increase compared with the baseline for IgG and a fourfold increase for IgA).