TABLE 2.

Primers used for deletion mutagenesis, site-directed mutagenesis, and sequencing

Primer name	Sequence (5′-3′)	Purpose
TM1-A-F	CGCGGATCCGGCGATGTTAATTATAATATGCTTAGTAAC	TM1 deletion
TM1-D-R	CCGGAATTCTATATGGAAGGAGAAATTAATGC	TM1 deletion
TM1-B-R	TTGTTCTCCTATAGTTTCTTTAGATTTAGTTAATGATTGGCTAAA	TM1 deletion
TM1-C-F	ACTAAATCTAAAGAAACTATAGGAGAACAAAATACAATAGAAAG	TM1 deletion
D48A-F	GGATTATATGTAATAGATAAAGGAGCTGGTTGGATATTAGGGGAACCC	D48A point mutation
D48A-R	CCTAATATACATTATCTATTTCCTCGACCAACCTATAATCCCCTTGGG	D48A point mutation
TrcSeq-1F	GGACAGCAAATCGGTCGG	Sequencing
2-F	GGTACCTTAAGCCAATCA	Sequencing
PH-310-F	GATTTAGCTGCTACAGAAAGA	Sequencing