FIG. 4.
Antagonistic effect of MPPL-1. (A) A total of 1 × 105 THP-1 cells were stimulated for 12 h with 1 or 10 nM FSL-1 in the presence or absence of 1 μM MPPL-1. Then the amounts of IL-6 released into the media were determined by ELISA. The results, expressed as means and standard deviations, are representative of three separate experiments. (B) HEK293 cells were transiently transfected with an NF-κB-driven firefly luciferase reporter plasmid together with the TLR-encoding plasmids indicated. The cells were stimulated for 6 h with 1 nM FSL-1 in the presence or absence of 100 nM MPPL-1. Then the cells were lysed, and the luciferase activity was measured. The results, expressed as means and standard deviations of values for triplicate wells, are representative of three separate experiments.