FIG. 5.

PKCζ inhibits the translocation of Akt to the membrane. Macrophages were cultured in coverslips, treated with or without LPS, and also pretreated with or without cell-permeative PKCζ inhibitor peptide (100 nM) for 1 h, followed by infection with L. donovani promastigotes (LD), as described in the legend to Fig. 1. After 24 h of culture, cells were stained with FITC-conjugated anti-Akt antibody and observed by confocal microscopy.