FIG. 4.

Kinetics of hepatic and splenic NKT cell subpopulations during primary P. yoelii infection in C57BL/6 and B6.CD1d^−/− mice. Numbers of (A) hepatic CD4⁺ NKT cells, (B) hepatic DN NKT cells, and (C) splenic CD4⁺ NKT cells in noninfected (day 0) B6 and B6.CD1d^−/− mice and at days 6 and 10 postinjection (p) of 4,000 sporozoites are shown. Cells were stained with anti-NK1.1, -TCRβ, -CD4, or -CD4⁺ CD8 mAbs, and the numbers of total (NK1.1⁺ TCRβ⁺), DN (CD4⁻ CD8⁻ NK1.1⁺ TCRβ⁺), and/or CD4⁺ (CD4⁺ NK1.1⁺ TCRβ⁺) NKT cells were determined by flow cytometry analysis. Data are representative of two independent experiments with four mice per time point. Results are expressed as mean values ± SD.