FIG. 5.

Numbers of hepatic and splenic invariant NKT cells in C57BL/6 mice during primary P. yoelii infection. Cells were stained with anti-NK1.1, -TCRβ, -CD4, or -CD4⁺ CD8 mAbs and CD1d tetramers (Tet) loaded with α-Galcer (α-galcer loaded CD1d Tet). Empty tetramers were used as controls. (A) Representative flow cytometry analysis of hepatic DN NKT cells (gated on TCRβ⁺ cells). (B) Numbers of total (CD4⁺ NK1.1⁺ TCRβ⁺) and Tet⁺ (α-Galcer-loaded CD1d Tet⁺ CD4⁺ NK1.1⁺ TCRβ⁺) CD4⁺ hepatic NKT cells as determined by flow cytometry analysis. (C) Numbers of total (CD4⁻ CD8⁻ NK1.1⁺ TCRβ⁺) and Tet⁺ (α-Galcer CD1d Tet⁺ CD4⁻ CD8⁻ NK1.1⁺ TCRβ⁺) DN hepatic NKT cells. (D) Numbers of total (CD4⁺ NK1.1⁺ TCRβ⁺) and Tet⁺ (α-Galcer-loaded CD1d Tet⁺ CD4⁺ NK1.1⁺ TCRβ⁺) CD4⁺ splenic NKT cells. Data are representative of two independent experiments performed with at least three mice per group. Results are expressed as mean values ± SD.